Figure 2
From: Discovery of transgene insertion sites by high throughput sequencing of mate pair libraries
The SOD1-G93A insertion site on MMU12. The SOD1-G93A transgene insertion site is at Chr12:97,165,800. In this region, there are no annotated coding sequences, nor is there functional non-coding sequence, as evidenced by the lack of evolutionarily conserved sequence. Instead, this region contains a number of low complexity LINE elements and simple repeats (A). Direct sequencing of the insertion site revealed the orientation of the last copy of the transgene in the multi-copy array with respect to the chromosome (B). Orientation of the chromosome is depicted proximal to distal (left to right) in both panels.