Transcriptomic analysis of differential host gene expression upon uptake of symbionts: a case study with Symbiodinium and the major bioeroding sponge Cliona varians

Table 1 de novo assembly data from the RNA-Seq experiments involving the three symbiont treatments “normal,” “reinfected,” and “aposymbiotic”

Dataset	N reads BT	GC content (%)	Sequence duplication (%)	N reads trimmed	Avg. L AT	N contigs	N bases (Mb)	Avg. L Contigs	Max contig L	N50
Normal	86,048,128	44	89/88.2	21,152,025	100.6	202,907	88.0	433.7	20,547	468
Aposymbiotic	71,135,240	43	82/81.5	13,527,103	100.7	142,371	67.3	473.2	33,836	556
Reinfected	39,036,828	42	89.5/89	9,003,053	100.6	51,020	21.7	417.1	5,732	454
Reference (pooled data)	157,183,368	-	-	34,679,128	100.7	292,182	87.1	468.3	21,891	502

The reference category represents pooled datasets from each of the other three. Abbreviations: N, number; BT, before trimming; Avg, average; L, length; AT, after trimming; Max, maximum. For the sequence duplication percentages, the first number refers to the forward reads (R1) and the second to the reverse reads (R2).