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Table 2 Results from suppressive subtractive hybridization experiments

From: Transcriptomic analysis of differential host gene expression upon uptake of symbionts: a case study with Symbiodinium and the major bioeroding sponge Cliona varians

Gene name

Insert size (bp)

E-value

Function

GO terms

Clones

3-hydroxybutyrate dehydrogenase type 2

270

8.00E-17

degradation of ketone bodies

metabolic process, catalytic activity

1

Actin-related protein 2/3 complex subunit

698

2.00E-65

cell locomotion & phagocytosis

cytoskeleton, protein binding

1

AP-2 complex subunit beta

672

3.00E-49

clathrin-mediated endocytosis

membrane, transport, protein binding

1

ATPase, H+ transporting, lysosomal, V0 subunit

359

1.00E-28

acidification control

ion transport, transport

1

Ca2+-triggered coelenterazine-binding protein 2

542

1.00E-14

calcium ion binding

calcium ion binding

1

Calcium-binding protein p22; Calcineurin

366

5.00E-47

calcium-dependent phosphatase

signal transduction

1

CHK1 checkpoint-like protein

203

3.00E-29

kinase activity in mitosis

protein kinase activity, nucleotide binding

1

Creatine kinase U-type, mitochondrial

511

3.00E-48

energy production and transport

nucleotide binding, transferase activity

1

Cyclophilin A

297

2.00E-50

calcium inhibition

protein folding, hydrolase activity

1

Cyplasin S

270

4.00E-08

Cell death induction

oxidoreductase activity

1

Cytoskeletal actin

330, 370

2.00E-60, 2.00E-58

cell motility & maintenance

cellular protein metabolic process,

2

Deleted in malignant brain tumors 1 protein-like; Scavenger receptor cysteine-rich type protein

669, 906

9.00E-34, 3.00E-32

removal of foreign substances

membrane

2

Dihydrolipoyl dehydrogenase

352

3.00E-31

mitochondrial glycine cleavage

cytoplasm, glycolysis

1

Dynein heavy chain

630

3.00E-60

cellular transport & maintenance

biological process, transferase activity

1

Ephrin type-B receptor 1; Protein tyrosine kinase

404

2.00E-17

developmental regulation

nucleotide binding, transferase activity

1

Ferritin

358 - 721

9.00E-86 - 1.00E-18

iron storage

ion binding

3

Ficolin-2

357

3.00E-29

innate immune recognition

signal transduction

1

G-protein gamma subunit

514

2.00E-04

signal transduction

signal transduction

1

Gamma-interferon-inducible lysosomal thiol reductase like

386

5.00E-23

macrophage activation

catalytic activity, biological process

1

Glutamine synthetase

316

7.00E-23

nitrogen metabolism

cellular nitrogen compound metabolic process

1

Heat shock protein 70

546

5.00E-24

protein folding & stress protection

response to stress

1

Hypothetical proteins

389 - 564

8.00E-24 - 2.00E-04

calcium absorption & metabolism

 

3

Inorganic pyrophosphatase

421

7.00E-41

lipid metabolism & calcium absorption

cytoplasm, ion binding

1

MafB chain A

428

5.00E-26

hematopoiesis regulation

transcription, cell death

1

Neurogenic locus notch protein homolog

617

5.00E-05

proliferative signaling

signal transduction

1

Nuclear pore complex Nup50

687

2.00E-29

intracellular protein transport

carbohydrate metabolic process

1

Proteasome subunit alpha

573

2.00E-26

processing of MHC class I peptides

cellular nitrogen compound metabolic process

1

Proteasome subunit beta

464

1.00E-64

intracellular protein degradation

cellular protein metabolic process, gene expression

1

Ribonuclease K-like; Salivary secreted ribonuclease

556

3.00E-14

degredation & protection

transport

1

Ribosomal proteins

223 - 383

3.00E-37 - 2.00E-04

translation machinery

translation, cellular protein metabolic processes, gene expression

8

RNA polymerase-associated protein LEO1

500

3.00E-07

histone methylation

protein binding, transcription

1

Selenoprotein Jb; J1a crystallin

637

1.00E-21

regulation of metabolism

hydrolase activity

1

Serum response factor

686, 709

9.00E-07, 6.00E-06

developmental regulation

cytoskeleton, signal transduction

2

Sulfide quinone reductase

259

5.00E-14

oxidation catalysis

oxidoreductase activity

1

Thymosin beta

295

2.00E-07

actin-sequestering protein

cytoskeleton

1

Tubulin alpha chain

283

2.00E-12

microtubule assembly

cytoskeleton

1

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein

482

2.00E-53

phosphoserine-binding for signal transduction

cytoplasm, protein targeting

1

Vacuolar sorting protein; sortilin-related receptor

789

1.00E-29

neuropeptide receptor activity & protein binding

membrane

1

von Willebrand factor A domain-containing protein-5a

507

2.00E-07

intracellular ligand interactions

transport

1

WAS protein family homolog 1

535

4.00E-11

nucleation promoting factor on endosomal surface

transport

1

  1. BLAST searches for each isolate (insert sizes shown) against NCBI database were used to determine gene identity. Gene function and GO categories were inferred from gene identity.