Comparative genomic analysis reveals distinct genotypic features of the emerging pathogen Haemophilus influenzae type f

Table 1 Laboratory strains and clinical Haemophilus influenzae isolates used in the present study

Strain	Serotype^a	MLST^b	Year of isolation	Isolation site^c	Underlying conditions^c	Clinical manifestation^d	Gender	Age	Geographical location of isolation^e	Reference
Laboratory strains
MinnA	b	n/a	1979	CSF	n/a	Septic shock	n/a	Child	Minneapolis, USA	[16]
3655	Nontypeable	n/a	n/a	Middle ear	n/a	AOM	n/a	Child	Missouri, USA	[17]
Clinical isolates
KR494	f	124	2008	Blood	Healthy	Septic shock	Male	70	Skåne	[9]
G19	f	124	2008	Blood	n/a	n/a	Male	57	Gothenburg	[3]
G20	f	124	2008	Blood	n/a	n/a	Male	4	Gothenburg	[3]
K238	f	124	2007	Blood	n/a	n/a	Female	63	Stockholm	[3]
L11	f	n/a	2006	Blood	MDS	Bacteremia/sepsis	Male	65	Skåne	[3]
L16	f	n/a	2007	CSF	Healthy	Bacteremia/sepsis	Male	74	Skåne	[3]
L21	f	124	2008	Blood	Healthy	Septic shock	Male	82	Skåne	[3]
L22	f	124	2008	Blood	Severe COPD	Bacteremia/sepsis	Male	81	Skåne	[3]
L24	f	124	2009	Blood	Healthy	Bacteremia/sepsis	Male	65	Skåne	[3]
L25	f	124	2009	Blood	Healthy	Septic shock	Male	51	Skåne	[3]
L29	f	124	2009	Blood	Healthy	Septic shock	Male	77	Skåne	[3]
L45	f	n/a	2005	Blood	Healthy	Septic shock	Male	51	Skåne	[3]
L50	f	124	2007	Blood	Healthy	Septic shock	Male	63	Skåne	[3]
L59	f	n/a	2007	Blood	Healthy	Severe sepsis	Male	71	Skåne	[3]
M1	f	124	2009	Blood	leucemia	Bacteremia/sepsis	Male	3	Skåne	[3]
M10	f	124	2008	Blood	Solid tumor	Bacteremia/sepsis	Female	77	Skåne	[3]
M14	f	124	2008	Blood	Solid tumor	Bacteremia/sepsis	Male	63	Skåne	[3]
M29	f	n/a	2006	CSF	Healthy	Septic shock	Male	0	Skåne	[3]
M54	f	n/a	1999	Blood	Healthy	Bacteremia/sepsis	Female	78	Skåne	[3]
S208	f	124	2008	CSF	n/a	n/a	Female	61	Stockholm	[3]
S229	f	124	2008	Blood	n/a	n/a	Female	88	Stockholm	[3]

^aIsolates were characterized by standard bacteriological techniques as described[3]. Strain serotype was verified by PCR whereas H. haemolyticus was excluded by 16S rRNA typing[3].
^bMultilocus sequence typing (MLST) was performed as previously described[18].
^cCOPD, chronic obstructive pulmonary disease; CSF, cerebrospinal fluid; MDS, myelodysplastic syndrome.
^dAOM, acute otitis media.
^eClinical isolates were from three different geographical areas of Sweden: Stockholm, Gothenburg and Skåne spanning from the years 1999–2009, isolated from blood or cerebrospinal fluid (CSF), but the clinical presentation, which was known for 15 of the 20 cases of invasive disease, ranged from Septic shock and epiglottitis to mild disease[3].
n/a, data not available.

ISSN: 1471-2164