Genome mapping and expression analysis of trans -encoded BTH_s1 and BTH_s39. (A) BTH_s1 overlaps with a sRNA prediction from Sipht (red) in the IGR between BTH_I0225 and BTH_I0226 (green). The yellow arrowhead bar maps the sequence boundaries defined by RACE. Probe intensities for BTH_s1 expression in B. thailandensis treated with (1) an antibiotics mixture containing sublethal concentrations of carbenicillin, chloramphenicol, erythromycin, and kanamycin (grey line), (2) the antibiotics mixure and 0.7 gm/L EDTA, a standard dosage for chelation therapy (blue line) or (3) untreated control (black line) are depicted. Predicted terminators are denoted as black triangles. (B) Northern blot of BTH_s1, BTH_s39, and BTH_I2030 demonstrates that these three genes are expressed as independent single transcripts. The size of the transcripts was determined on a 15% denaturing polyacrylamide gel using a biotinylated RNA ladder. (C) BTH_s39 overlaps with a sRNA prediction from Sipht (red) in the IGR between BTH_I2030 and BTH_I2031 (green). The yellow arrowhead bar maps the sequence boundaries defined by RACE. Probe intensities for BTH_s39 expression in B. thailandensis for the three different conditions and predicted terminators are denoted as described in (A).