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Table 1 Burkholderia growth conditions for microarray analysis

From: Differential expression of small RNAs from Burkholderia thailandensis in response to varying environmental and stress conditions

Time course experiments
ID Medium Conditions OD600 Time pt Time from t0 Label
1 Nutrient broth 25 C 1 0   temp t0
2 Nutrient broth 37 C 0.65 1 20m temp t1
3 Nutrient broth 37 C 1 2 63m temp t2
4 Nutrient broth 37 C 1.25 3 78m temp t3
5 Nutrient broth 37 C 1.5 4 96m temp t4
6 Nutrient broth pH 9 0.6 0   pH t0
7 Nutrient broth pH 4 0.7 1 15m pH t1
8 Nutrient broth pH 4.5 1 2 15h pH t2
9 Nutrient broth pH 4.5 1.2 3 15.6h pH t3
10 Nutrient broth pH 5+ 1.6 4 15.3h pH t4
11 Nutrient broth no salt 0.6 0   salt t0
12 Nutrient broth 350mM NaCl 0.7 1 15m salt t1
13 Nutrient broth 350mM NaCl 1 2 107m salt t2
14 Nutrient broth 350mM NaCl 1.2 3 147m salt t3
15 Nutrient broth 350mM NaCl 1.5 4 201m salt t4
16 M9 succinate 80mM PO4 1 0   PO4 t0
17 M9 succinate 40mM PO4 0.6 1 15m PO4 t1
18 M9 succinate 40mM PO4 0.9 2 97m PO4 t2
19 M9 succinate 40mM PO4 1.2 3 160m PO4 t3
20 M9 succinate 20mM PO4 0.6 4 188m PO4 t4
21 M9 succinate 20mM PO4 0.6 5 363m PO4 t5
Five replicates for all conditions, except samples at pH 4.5 with 3 replicates.
Single time point experiments
ID Medium Temp Replicates Description Label
22 M9 succinate 16 4 temp 16 C 16°C
23 M9 succinate 37 4 temp 37 C 37°C
24 M9 succinate 37 1 70 mM phosphate PO4
25 M9 succinate 37 1 40 mM phosphate low PO4
26 M9 succinate 37 1 anaerobic N
27 M9 succinate 37 1 anaerobic + CO2 N + CO2
28 M9 succinate 37 1 nitrogen-limited low N
29 M9 succinate 37 1 sulfur-limited low sulfur
30 M9 succinate 37 1 solid media solid
31 M9 succinate 37 1 50 mM Mg++ high Mg
32 M9 succinate 37 1 5 uM Mg++ low Mg
33 M9 succinate 37 1 0.07g/L EDTA EDTA
34 M9 succinate 37 1 0.07g/L EDTA with nutrient-limit EDTA/limit
35 M9 succinate 37 1 +0.1% phenol phenol
36 M9 succinate 37 1 +200 ppm Bleach bleach
37 M9 succinate 37 1 +5% EtOH ethanol
38 M9 glucose 25 1 M9-glucose glucose
39 M9 galactose 37 1 M9-galactose galactose
40 M9 proline 37 1 M9-proline proline
41 Luria broth 25 4 temp 25 C 25°C
42 Luria broth 37 4 temp 37C 37°C
43 Luria broth 41 4 temp 41 C 42°C
44 Luria broth 37 4 pH 9 pH 9
45 Luria broth 37 4 pH 5 pH 5
46 Luria broth 37 4 80mM Peroxide H202
47 Luria broth 37 2 0.5M NaCl NaCl
48 Calf serum 37 4 CS infusion, temp 37 C 37°C
49 Calf serum 37 1 antibiotics antibiotics
50 Calf serum 37 1 antibiotics + 0.07g/L EDTA antibiotics + EDTA
51 Calf serum 37 1 aspirin salicylate
52 Brain heart 37 1 solid media solid
53 Tryptic soy 37 1 kan mutant kan mutant
54 Tryptic soy 37 1 wt wt
  1. The antibiotics treatment consisted of carbenicillin (100 μg/ml), chloramphenicol (30 μg/ml), erythromycin (200 μg/ml, and kanamycin (50 μg/ml). The EDTA concentration of 0.07g/L is standard usage for chelation therapy. The kan mutant (ID53) is a spontaneous B. thailandensis mutant isolated in response to a sublethal exposure to kanamycin.