Integrated transcriptome analysis of mouse spermatogenesis

BMC Genomics

Table 1 Expression profiles of selected genes associated with meiosis

Gene	Temporal 1-8	Schultz’03 1-8	Shima’04 A-E	Chalmel’07 SO,MI,ME,PM	Deconvolution A-E
*Aym1*	6	5	D	ME	D
*Brca1*	2	2	-	-	B
*Brca2*	3	6	-	-	B
*Dazl*	3	4	-	ME	B
*Dmc1*	3	4	-	PM	B
*Fancm* ^§	3	-	-	-	B
*Itga6*	1	2	-	MI	A
*Itgb1*	2	2	A	SO, MI	B
*Mei1* ^§	3	-	-	-	B
*Mei4* ^§	3	-	-	-	-
*Mlh1* ^§	3	-	-	-	C
*Mnd1*	3	3	A	-	-
*Mre11a* ^§	Const	-	-	-	C
*Msh4*	4	6	D	PM	C
*Nbn*	Const	2	-	-	B
*Pms2*	3	3	C	-	C
*Prdm9* ^§	3	-	-	-	B
*Psmc3ip*	6	7	D	ME	C
*Rad21*	2	-	-	MI	B
*Rad50*	3	2	B	-	B
*Rad51*	3	3	-	MI	B
*Rad51c*	Const	5	D	ME	D
*Rad51l1*	Low	6	-	-	-
*Rad51l3* ^§	3	-	-	-	C
*Rad52*	3	6	D	-	C
*Rad54l* ^§	3	-	-	-	B
*Rec8*	5	5	E	PM	E
*Rpa1*	5	5	D	-	D
*Spag4l*	8	8	E	PM	E
*Spata22* ^§	4	-	-	-	C
*Spo11*	6	5	D	ME	C
*Stra8*	3	4	C	MI	B
*Sun1*	Const	-	-	-	C
*Sycp1*	4	4	C	ME	C
*Sycp2*	4	-	-	ME	C
*Sycp3*	4	-	-	ME	C
*Tex11*	3	-	-	MI	B

While Dmc1 and Mnd1 are found in our cluster 3 – see Figure 2 – they are either misclassified, or unclassified by the microarray-based study of Shima et al. [9] and Chalmel et al. [6]. Note that there were no Affimetrix probes in the regions of the Spata22 gene and Prdm9 gene (in [8] and [9]). Genes marked with (^§) have not been either interrogated or classified in the microarray studies [6, 8, 9].

ISSN: 1471-2164