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Figure 1 | BMC Genomics

Figure 1

From: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells

Figure 1

Assessment of the TLDA reproducibility. The TLDA reproducibility was assessed by testing, from one RNA sample, two separate qPCR amplifications from A- the same RT and pre-amplification product (RT1-d1 vs RT1-d2: Bland-Altman) and B- separate RT and pre-amplification reactions (RT1 (d1) vs RT2: Bland-Altman). RT with 150 ng of RNA isolated from 3×106 cells with Macherey-Nagel kit. Only miRNAs with Ct < 32 were considered.

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BMC Genomics

ISSN: 1471-2164

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