Gene expression profiling in putative pig ICM cells. (A) Venn diagram of ICM-specific gene expression of mouse and pig embryos derived in vivo and pig embryos derived in vitro. ICM-specific genes were found to be expressed at higher levels than genes expressed in the blastocyst of the TE sample when corrected for P-value <0.05, fold change >2, and Blast RPKM >3. (B) Venn diagram of genes expressed in the mouse, pig and human ICM . (C) Pou5f1, Nanog, Gata6 and Cdx2 expression levels were measured using RPKM values and RNA-sequencing results. (D) Pou5f1 immunostaining at different stages of porcine PED.