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Table 6 GO enrichment analysis of the most variable genes in granulosa cells from small healthy follicles using GOEAST

From: Transcriptome profiling of granulosa cells from bovine ovarian follicles during atresia

Biological process Molecular function Cellular component Symbol Entrez gene name
Regulation of vascularity Angiogenesis (P = 0.009)    CYR61 cysteine-rich angiogenic inducer
COL18A1 collagen 18
THBS1 thrombospondin 1
CXCR4 chemokine receptor 4
ANGPT2 angiopoietin 2
VEGFA vascular endothelial growth factor A
Energy metabolism Glycogen biosynthetic process (P = 0.044) Insulin-like growth factor binding, two component sensor activity microsome ISR2 insulin receptor 2
EGFR epidermal growth factor receptor
HSP90AA1 heat shock protein 90 kDa alpha (cytosolic), class A member 1
PDK4 pyruvate dehydrogenase kinase, isozyme 4
IGFBP2 insulin-like growth factor binding protein 2
CTGF connective tissue growth factor
CYR61 cysteine-rich angiogenic inducer
MGST1 glutathione transferase 1
CALR calreticulin
PTGES prostaglandin E synthase
PLC4 phospholipase C, beta 4
OPTN optineurin
ASPSCR1 alveolar soft part sarcoma chromosome region, candidate 1
Cell Cycle Mitosis (P = 2.3 × 10-5)   Condensed chromosome kinetochore, spindle CCNA2 cyclin A2
CCNB1 cyclin B1
CCNB2 cyclin B2
UBE2 ubiquitin conjugating enzyme 2
CDK1 cyclin-dependent kinase 1
NUSAP nucleolar and spindle associated protein 1
SKA1 spindle and kinetochore associated complex subunit 1
NUP85 nucleoporin 85 kDa
CENPN centromere protein N
CENPO centromere protein O
ZW10 kinetochore associated homolog
BUB1 budding inhibited by benzimidazoles 1 homolog (yeast)
STK serine/threonine kinase
Extracellular matrix Extracellular matrix organisation (P = 0.01) Heparin binding, extracellular matrix binding Basement membrane COL1A1 collagen type I, alpha 1
COL1A2 collagen type I, alpha 2
COL3A1 collagen type III, alpha 1
COL4A3 collagen type IV, alpha 3
COL4A4 collagen type IV, alpha 4
COL18 collagen type XVIII
LAMB1 laminin, beta 1
VCAN versican
FMOD fibromodulin
ADAMTS1 ADAM metallopeptidase with thrombospondin type 1 motif, 1
ADAMTS6 ADAM metallopeptidase with thrombospondin type 1 motif, 6
MMP2 matrix metallopeptidase 2
TIMP1 TIMP metallopeptidase inhibitor 1
OGN osteoinductive factor/osteoglycin
OSF-2 osteoblast-specific factor 2
SPARCL1 SPARC-like 1 (hevin)
ASPN asporin
Inflammation   Fibrinogen complex THBS1 thrombospondin 1
NOS biosynthesis (P = 0.044) CALR calreticulin
Antigen processing (P = 0.026) BOLA-A major histocompatibility complex (MHC), class I, A
TGFβ receptor signalling pathway (P = 0.05) BOLA-N MHC class I antigen
Fibrinolysis (P = 0.09)
Cell migration    CXCR4 chemokine receptor 4
Axon guidance (P = 0.0008) ABLIM1 actin binding LIM protein 1
COL18 collagen type XVIII
EFNA5 ephrin A5
THBS1 thrombospondin 1
EGFR epidermal growth factor receptor
TDGF teratocarcinoma-derived growth factor 1
F2RL1 coagulation factor II (thrombin) receptor-like 1
INSR insulin receptor
MAPK activity (P = 0.002)    THBS1 thrombospondin 1
TDGF teratocarcinoma-derived growth factor 1
    INSR insulin receptor
  1. †The significance of association for the GO enrichment analysis was determined by the Benjamini-Yekuteli FDR method for multiple comparisons. The variability of expression was determined by a frequency distribution of the coefficients of variation for probe sets across the arrays in the small healthy follicle group. The cut-off chosen was a coefficient of variation of > 46.8% (n = 10 arrays, n = 682 probe sets).