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Table 9 Primers and conditions used for quantitative RT-PCR

From: Transcriptome profiling of granulosa cells from bovine ovarian follicles during atresia

Target

Primer sequences, 5′-3′

GenBank accession number

PCR reaction conditions

CYP17A1

forward accatcagagaagtgctccgaa

NM_174304

2 min 50°C, 10 min 95°C, 40 × cycles of 15 s 95°C and 60 s 60°C

reverse ccacaacgtctgtgcctttgt

18S

forward agaaacggctaccacatccaa

DQ2224

2 min 50°C, 10 min 95°C, 40 × cycles of 15 s 95°C and 60 s 60°C

 

reverse cctgtattgttatttttcgt

 Â