Figure 7From: Differential gene expression between functionally specialized polyps of the colonial hydrozoan Hydractinia symbiolongicarpus (Phylum Cnidaria)Diagram illustrating approach taken for adjusting for gametic expression. DE analysis was conducted between male and female gonozooid libraries (step 1), identifying significantly up- or down-regulated transcripts (step 2). Those transcripts found to be significant were excluded from the template pool and a DE analysis on those non-significant transcripts included counts from both male and female libraries (step 3 shown in black). Then, a second DE analysis was conducted on transcripts up-regulated in female gonozooids. For these comparisons, only the expression counts from male gonozooids libraries were used (step 3 shown in red). Results from the both analyses (step 3) were combined.Back to article page