Figure 1

Design of TALENs that target the BGIBMGA000702 gene. (a) Schematic representation of the silkworm chromosome 1 and position of BmBlos2 gene and Bm702 gene. Brown box represents the entire chromosome and blue boxes below represent scaffolds mapped to chromosome one. The position of BmBlos2 gene and Bm702 gene are indicated by oblique lines. Exon 2 of each gene is indicated by a black box. Blue and red arrows represent the position of TALEN recognition sites for BmBlos2 gene and Bm702 gene, respectively. Black arrows and letters below them represent the position and names of primers used to detect mutations. (b) Schematic representation of TALEN proteins. A triple flag tag (light green boxes) and a nuclear localization signal (NLS) (purple boxes) are fused to TALE domain (yellow boxes) of each TALEN monomer. The Fok I domain (red boxes) is linked to the TALE domain through a flexible linker. (c) TALEN target sequences and the corresponding RVDs within each repeat. The entire TALEN recognition sequence is shown on the top, in which underlined sequence represents the recognition site of the corresponding TALEN monomer. RVDs utilized for each TALEN monomer are shown on the bottom. The number at the top of the left panel indicates the position of the repeat. The letters at the top of each TALEN monomer represent the target sequence and the letters below represent the RVDs of the corresponding repeat.