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Figure 2 | BMC Genomics

Figure 2

From: Multiplex genomic structure variation mediated by TALEN and ssODN

Figure 2

Genome editing activity of TALEN-702. (A) In vivo analysis of TALEN activity in HEK 293 T cells using a luciferase reporter system. The entire TALEN-702 recognition site was cloned into a pSSA-Luc vector and transfected into HEK 293 T cell with (TALEN-702) or without (control) in vitro transcribed TALEN-702 mRNA. Luciferase activity was detected 24 hours post transfection. (B) Transient assay of TALEN activity in silkworm embryos using T7EI assay. PCR was performed using primer 702 F-883 and 702R-611 using genomic DNA from un-injected embryos (lane 2 and 3) or injected embryos (lane 4 and 5). M represents DNA ladders, the letters on the left indicate length of each band.

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