Screenshot of the web interface. The web interface is divided into three main parts: data upload and options section, results section, and phylogenetic tree section (not shown). A) Example data were uploaded and processed with default parameters. B) The results section is split into a summary and a section listing each reference protein and a detailed analysis of each predicted protein down to single CUG codons. For every reference protein, the predicted gene and, if applicable, the respective CUG positions are shown. For every predicted CUG position, which could be mapped onto the reference data, the amino acid composition and CUG codon usage at the respective positions in the reference data are listed. The predicted actin related protein class 4 (Arp4) contains one CUG at position 163. This position corresponds to alignment position 291 in the reference alignment. It is here indicated by a black box. All CUG codons are noted as leucine in the predicted sequence, regardless the suggested codon usage.