Longissimus dorsi transcriptome analysis of purebred and crossbred Iberian pigs differing in muscle characteristics

Table 2 Technical validation of microarray results by qPCR

Gene	Ratio microarray (DU×IB)/IB	PP valuemicroarray	ratio qPCR (DU×IB)/IB	P valueqPCR	Correlation ( r)	P value (H _o :r = 0)
IGF2	4.88	<0.00001	2.95	0.0001	0.796	<0.0001
KERA	3.41	0.00001	2.41	0.0009	0.889	<0.0001
FMOD	3.35	0.00006	2.78	0.0048	0.764	<0.0001
COL1A1	2.18	0.00082	2.15	0.0060	0.540	0.0030
FBN2	1.88	0.00009	1.76	0.012	0.744	<0.0001
AEBP1	1.81	0.00111	2.00	0.019	0.832	<0.0001
LOX	1.72	0.00071	1.58	0.019	0.506	0.0084
FKBP14	1.72	0.00005	1.60	0.025	0.794	<0.0001
PSMD11	0.73	0.00541	0.65	0.046	0.444	0.0229
ALOX5AP	0.70	0.00139	0.78	0.128	0.189	0.3331
CASP4	0.64	0.00216	0.54	0.084	0.869	<0.0001
ELOVL6	0.64	0.00114	1.11	0.561	0.086	0.6616
NFKBIZ	0.59	0.00369	0.82	0.358	0.502	0.0065
ME1	0.50	0.00028	0.57	0.024	0.802	<0.0001
PLA1A	0.42	0.00017	0.42	0.074	0.769	<0.0001
PON3	0.31	<0.00001	0.37	0.001	0.787	<0.0001
SCD	0.65	0.15186	0.50	0.030	0.866	<0.0001
ELOVL5	1.07	0.28406	1.13	0.433	0.255	0.2089

ISSN: 1471-2164