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Table 2 Technical validation of microarray results by qPCR

From: Longissimus dorsi transcriptome analysis of purebred and crossbred Iberian pigs differing in muscle characteristics

Gene Ratio microarray (DU×IB)/IB PP valuemicroarray ratio qPCR (DU×IB)/IB P valueqPCR Correlation ( r) P value (H o :r = 0)
IGF2 4.88 <0.00001 2.95 0.0001 0.796 <0.0001
KERA 3.41 0.00001 2.41 0.0009 0.889 <0.0001
FMOD 3.35 0.00006 2.78 0.0048 0.764 <0.0001
COL1A1 2.18 0.00082 2.15 0.0060 0.540 0.0030
FBN2 1.88 0.00009 1.76 0.012 0.744 <0.0001
AEBP1 1.81 0.00111 2.00 0.019 0.832 <0.0001
LOX 1.72 0.00071 1.58 0.019 0.506 0.0084
FKBP14 1.72 0.00005 1.60 0.025 0.794 <0.0001
PSMD11 0.73 0.00541 0.65 0.046 0.444 0.0229
ALOX5AP 0.70 0.00139 0.78 0.128 0.189 0.3331
CASP4 0.64 0.00216 0.54 0.084 0.869 <0.0001
ELOVL6 0.64 0.00114 1.11 0.561 0.086 0.6616
NFKBIZ 0.59 0.00369 0.82 0.358 0.502 0.0065
ME1 0.50 0.00028 0.57 0.024 0.802 <0.0001
PLA1A 0.42 0.00017 0.42 0.074 0.769 <0.0001
PON3 0.31 <0.00001 0.37 0.001 0.787 <0.0001
SCD 0.65 0.15186 0.50 0.030 0.866 <0.0001
ELOVL5 1.07 0.28406 1.13 0.433 0.255 0.2089