Longissimus dorsi transcriptome analysis of purebred and crossbred Iberian pigs differing in muscle characteristics

Óvilo, Cristina; Benítez, Rita; Fernández, Almudena; Núñez, Yolanda; Ayuso, Miriam; Fernández, Ana Isabel; Rodríguez, Carmen; Isabel, Beatriz; Rey, Ana Isabel; López-Bote, Clemente; Silió, Luis

doi:10.1186/1471-2164-15-413

Table 6 Information on the primer pairs used for quantitative real-time PCR analysis

From: Longissimus dorsi transcriptome analysis of purebred and crossbred Iberian pigs differing in muscle characteristics

Gene symbol	Gene name	Genbank Acc. number	Primer sequences 5′-3′	Amplicon size (bp)	Efficiency (%)
IGF2	Insulin-like growth factor 2	NM213883	GCCGCTGCTCGTGCTGCTCGTCTT	151	86
IGF2	Insulin-like growth factor 2	NM213883	GCTTGCCGGCCTGCTGAA	151	86
KERA	Keratocan	XM001927128	GTGGCCTTCCTGAGACTAAACC	198	89
KERA	Keratocan	XM001927128	AGGGCATATCACAGAGACATTCAC	198	89
FMOD	Fibromodullin	XM003130105	GCTGCTATATGTGCGGCTGTC	194	93
FMOD	Fibromodullin	XM003130105	AGAAACTGCTAATGGAGAACT	194	93
COL1A1	Collagen alpha-1	EF136662	AGCCCAGCGTGCCCCAGAAGAA	164	88
COL1A1	Collagen alpha-1	EF136662	ACATCAGGCGCAGGAAGGTCAGC	164	88
FBN2	Fibrillin 2	XM003123897	GGACGCTGCATACCTACTGT	201	96
FBN2	Fibrillin 2	XM003123897	AATGCATGCTTGCTTGGTAGG	201	96
AEBP1	AE binding protein 1	XM003134886	CGGCGGCATGGGCATCGTCAAC	233	90
AEBP1	AE binding protein 1	XM003134886	TGCCCTGCTCGTCCGTCACTACCC	233	90
LOX	Lysyl oxidase	NM001206403	CTGAGATGCGCTGCGGAGGAAAAC	223	88
LOX	Lysyl oxidase	NM001206403	TGGCATCAAGCAGGTCGTAGTGG	223	88
FKBP14	FK506 binding protein 14	XM005673279	TTCCGGAACTTCTTTCCTGCTCT	250	91
FKBP14	FK506 binding protein 14	XM005673279	GGCTGACCATTGTTATGTTTGTGA	250	91
PSMD11	Proteasome 26S subunit, non-ATPase	XM003131741	TCTTACGCCAGGCTTTGGAG	219	91
PSMD11	Proteasome 26S subunit, non-ATPase	XM003131741	CTGTGGTTCGAGCAGAGGTT	219	91
ALOX5AP	Arachidonate 5-lipoxygenase-activating protein	NM001164001	TGGACTGATGTACCTGTTTGTGAG	213	94
ALOX5AP	Arachidonate 5-lipoxygenase-activating protein	NM001164001	AGAGGGGAGACGGTGGTGGTGA	213	94
CASP4	Caspase 4	XM003129812	AATATGCTTGGCGCTGTCAC	190	97
CASP4	Caspase 4	XM003129812	TGGTGCTTCTCGAAGTTGGT	190	97
ELOVL6	Fatty acid elongase 6	XR305072	AGAACACGTAGCGACTCCGAAGAT	182	96
ELOVL6	Fatty acid elongase 6	XR305072	GACATGCCGACCGCCAAAGATAA	182	96
NFKBIZ	NF-kappa-B inhibitor zeta-like	XM003132694	TATGATGGCCTGACTCCTCTACAC	196	91
NFKBIZ	NF-kappa-B inhibitor zeta-like	XM003132694	TGCGGCCACTTTTACGAT	196	91
ME1	Malic Enzyme	XM001924333	TTTCCTGGAGTTGCCCTTGGTGT	213	90
ME1	Malic Enzyme	XM001924333	GGTGGCTGTCTTTTCTTGGTATGC	213	90
PLA1A	Phospholipase A1 member A	XM003483312	TGTGGGCAGCTAGTGGAAGAAAGT	215	91
PLA1A	Phospholipase A1 member A	XM003483312	TCCACGGCTGAAAAGTAGACACC	215	91
PON3	Paraoxonase 3	HQ542303	ACGGGAGATATTTGGGCAGG	142	92
PON3	Paraoxonase 3	HQ542303	TGTTGGCATACTCGGTGCTT	142	92
SCD	Stearoyl-CoA desaturase	JN613287	TCCCGACGTGGCTTTTTCTTCTC	205	90
SCD	Stearoyl-CoA desaturase	JN613287	CTTCACCCCAGCAATACCAG	205	90
ELOVL5	Fatty acid elongase 5	ENSSSCG00000024149	CTTGCCGGGGGATTTTGGTTG	223	82
ELOVL5	Fatty acid elongase 5	ENSSSCG00000024149	TTGCGCAGGATGAAGAAGAAGGTG	223	82
DLK1	Delta-like 1 homolog	NM_001048187	CGGGCCCTGCGTGATGAATGG	208	83
DLK1	Delta-like 1 homolog	NM_001048187	AGGGCAGCGGCAGCGGAAGTC	208	83

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ISSN: 1471-2164

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