Figure 2

Northern blot detection of the sRNAs in Ea1189 and Ea1189Δ hfq at 6 and 12 hrs post-inoculation in Hrp-inducing minimal medium. 5S rRNA was used as the loading control. A biotin-labeled RNA marker was used to estimate the sizes of the sRNAs. sRNA Hrs5, Hrs6, Hrs8, Hrs10, Hrs12, Hrs13, Hrs19, Hrs27 have only one major band whereas sRNA Hrs1, Hrs9, Hrs21, and Hrs31 have two major bands.