Figure 1

Number and types of differentially expressed miRNAs in different sources of placenta together with their relation to global methylation status and expression of miRNA processing genes. (A) Venn diagram of the distribution and the number of differential miRNA expression between and specific to different types of day-50 placentas. Each circle represents the number of differentially expressed miRNAs between two placenta types out of 377 analyzed miRNAs. For example, NT vs. AI represents the number of differentially regulated miRNAs (↓ denotes down regulation and ↑ denotes upregulation) in the NT placentas compared to that of AI. (B) Hierarchical cluster (log2 fold change) of NT, IVP and AI day-50 placentas compared to each other and characterization of differentially expressed X-linked miRNAs. (C) Family wise differential expression of miRNAs in different sources of day-50 placentas. Log2 fold change of 43 miRNAs belong to 9 miRNA families which were deregulated in different sources of placenta were plotted to visualize the family wise expression differences in the placentas of different sources of pregnancies. (D) Global DNA methylation (%) in day-16 elongated embryos and day-50 placenta of different sources of pregnancy. (E) Significant differential expression of 10 miRNA processing genes in different sources of placentas and donor fibroblast cells.