Figure 1

Quality and features of the RNA-seq datasets obtained in the current study. (A) Distribution of the RNA-seq reads along annotated Arabidopsis genomic features. Among reads that unambiguously match the Arabidopsis genome, more than 97% reads match annotated exons. (B) Relation between the RNA-seq read coverage and the length of the transcriptional unit. The x-axis indicates the relative length of the transcripts, and the y-axis shows the median depth of coverage. (C) Saturation curve for gene detection. Randomly sampled reads are plotted against the expressed genes. The x-axis shows the number of the mapped reads and the y-axis displays the number of the expressed genes. (D) Transcription profiles are plotted across the Arabidopsis genome. The distribution of the RNA-seq read density along the chromosome length is shown. Each vertical blue bar represents log2 of the frequency of reads plotted against the chromosome coordinates. A schematic drawing of the chromosome and its features is shown below the read density. Approximate boundaries of Arabidopsis centromeres are depicted in violet.