Figure 6

Eight AS events in six SR genes validated by RT-PCR and visualized by the IGV browser. In the RT-PCR validation, the grey asterisk (*) to the right side denotes the alternative splice form. The red arrow on top indicates that an increase (pointing upward) or decrease (pointing down) in AS events is exhibited at that salt concentration. In the schematic exon-intron structure below each gel picture, the blue bars represent exons and the red bars represent splice junctions. The green arrows indicate primers designed for RT-PCR validation. In the IGV visualization, the exon-intron structure of each gene is given at the bottom of each panel. The grey peaks above the exon-intron structure indicate the RNA-seq read density across the gene. The red arrows represent alternative splice sites. The blue arcs indicate splice junction reads that support the junctions. 5'SS, alternative 5' splice site; 3'SS, alternative 3' splice site.