Figure 3
S. kudriavzevii presents increased translation efficiency at low temperatures. In panel A, the inhibitory effect of the translation inhibitor paromomycin was evaluated by measuring the halo diameter generated in S. cerevisiae (T73) or S. kudriavzevii (CR85) lawns growing in GPY plates at 28 or 12°C. In panel B, the translation efficiency was evaluated by measuring 35S-Methionine incorporation 16 h after transfer S. cerevisiae (Q23) or S. kudriavzevii (CR85) cells to cold (12°C) rich media. Cpm values were normalized with OD600, relativized to maximum value and represented against the time. Average of biological triplicates and standard deviations are shown.