Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques

BMC Genomics

Table 3 Genes analyzed by RT-qPCR

Gene	Chromosome	Primer sequence		Amplicon length (bp)	Accession number
ACTB	3	F:	GTTGCGTTACACCCTTTCTTG	146	NM_001033084.1
		R:	CTGTCACCTTCACCGTTCC
GAPDH	11	F:	CCTGCACCACCAACTGCTTA	74	NM_001195426.1
		R:	CATGAGTCCTTCCACGATACCA
AKR1C1	9	F:	CCGCCATATTGATTCTGCTCAT	132	NM_001195574.1
		R:	TGGGAATTGCACCAAAGCTT
HBB	14	F:	GTCCTCTCCTGATGCTGTTATG	102	NM_001164428.1
		R:	TTGAGGTTGTCCAGGTGATTC
NCAM1	14	F:	GAGCAAGAGGAAGATGACGAG	150	XM_001083697.2
		R:	GACTTTGAGGTGGATGGTCG
NR4A2	12	F:	CCAGTGGAGGGTAAACTCATC	145	NM_001266910.2
		R:	AGGAGAAGGCAGAAATGTCG
USP16	3	F:	GCAGAACTTGTCACAAACACC	146	NM_001260999.2
		R:	CTAAAGTAAGAGGGCCTGGAG
SAP18	17	F:	GGAAATGTACCGTCCAGCGA	109	NM_001261034.1
		R:	TGCCCTTCTTTCTAGCTTCTGG
SERPINA3	7	F:	GCTGGGCATTGAGGAAGTCT	123	NM_001195350.1
		R:	GTGCCCTCCTCAGACACATC
HBA2	20	F:	CGACAAGAGCAACGTCAAGG	126	NM_001044724.1
		R:	TCGAAGTGGGGGAAGTAGGT
IRF3	19	F:	TGGGTTGTGTTTAGCAGAGG	90	NM_001135797
		R:	GAAAAGTCCCCAACTCCTGAG
APOC1*	19	F:	TTCTGTCGATGGTCTTGGAAG	138	AK240617.1
		R:	CACTCTGTTTGATGCGGTTG
TTR	18	F:	TCACTTGGCATCTCCCCATTC	114	NM_001261679
		R:	GGTGGAATAGGAGTAGGGGCT

Primers (F: forward and R: reverse) used for gene amplification, amplicon length and GenBank® accession numbers of the macaque cDNA sequences used for primer design. All primers were designed according to the genome sequence of Macaca mulatta.
*Apolipoprotein C-I (APOC1) primers were designed according to the genome sequence of Macaca fascicularis because the Macaca mulatta mRNA sequence was not annotated (TSA Macaca mulatta Mamu_450725, accession number: JV045807.1). Homology between the two sequences was 99%.

ISSN: 1471-2164