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Table 4 RT-qPCR confirmation of microarray results

From: Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques

Gene symbol

Microarray fold change

RT-qPCR fold change

    

SYBR® Green

TaqMan®

 

Min

Max

Mean

FC

P value

FC

P value

AKR1C1

2.3

2.9

2.5

1.7

0.433

2.4

0.235

HBB

−2.2

−2.6

−2.4

0.2

0.020

0.3

0.021

NCAM1

−1.1

2.5

−0.3

0.5

0.160

-

-

NR4A2

1.1

−2.1

−1.6

0.4

0.248

-

-

USP16

−1.2

−5.5

−2.6

0.5

0.308

-

-

SAP18

−1.2

−2.6

−1.7

0.8

0.393

-

-

SERPINA3

10.0

16.0

13.0

18.7

0.0001

15.3

0.0005

HBA2

-

-

-

0.2

0.041

0.2

0.019

IRF3

2.0

2.1

2.0

1.3

0.123

-

-

APOC1

4.3

-

4.3

6.3

0.047

6.8*

0.028*

TTR

3.1

-

3.1

7.1

0.025

5.9

0.076

  1. Differential expression of selected genes analyzed by microarray and RT-qPCR. For microarray analysis, the lowest (Min), the highest (Max) and the average (Mean) fold change values of all the respective probes are shown. For RT-qPCR analysis, fold change (FC) and statistical significance (p-value) for both SYBR® Green and TaqMan® results are shown. In bold are the genes validated with statistical significance. HBA2 was not present in the array chip.
  2. *Normalization performed vs. ACTB only.
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BMC Genomics

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