CD133- and CD133+ liver cancer cells differentially express genes involved in DNA methylation establishment and maintenance. a. Liver cancer cell lines (Huh7, and HepG2) were assessed for surface expression of CD133 by flow cytometry. The left panel shows a representative histogram for each of the cell lines (black histogram), with background (secondary antibody) represented by the empty histogram (logarithmic scale). The average expression +/- SD from three assays is shown in the right panel. b. The same cell lines were sorted using MACS (as described in Methods) and RNA was extracted to measure the expression levels of the genes involved in DNA methylation or demethylation. Expression was normalized to the housekeeping gene GAPDH. (*) indicates P value < 0.05.