Figure 4From: Efficient de novo assembly of large and complex genomes by massively parallel sequencing of Fosmid poolsDiscovery of novel regions of the genome by recurrent sampling with Fosmid pools. The diploid whole genome shotgun (WGS) assembly was used as a reference. All the available 972 FPs assemblies were aligned against the WGS assembly. Only contigs longer than 1000 bp and alignments longer than 500 bp (percent identity >99%) were counted. BLAST mapping masked WGS sequences at discrete points every 500 bp along the contig coordinates. Cumulative statistics were collected in random FP order. Upper panel: Total assembled length of FP (horizontal axis) versus the summary length of WGS contigs having matches to FP contigs (vertical axis). Lower panel: Fraction of current FP contig length mapped to WGS regions which had not been found (i.e. not masked) in previous FP assemblies.Back to article page