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Figure 4 | BMC Genomics

Figure 4

From: Efficient de novo assembly of large and complex genomes by massively parallel sequencing of Fosmid pools

Figure 4

Discovery of novel regions of the genome by recurrent sampling with Fosmid pools. The diploid whole genome shotgun (WGS) assembly was used as a reference. All the available 972 FPs assemblies were aligned against the WGS assembly. Only contigs longer than 1000 bp and alignments longer than 500 bp (percent identity >99%) were counted. BLAST mapping masked WGS sequences at discrete points every 500 bp along the contig coordinates. Cumulative statistics were collected in random FP order. Upper panel: Total assembled length of FP (horizontal axis) versus the summary length of WGS contigs having matches to FP contigs (vertical axis). Lower panel: Fraction of current FP contig length mapped to WGS regions which had not been found (i.e. not masked) in previous FP assemblies.

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