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Figure 1 | BMC Genomics

Figure 1

From: Fragmented mitochondrial genomes of the rat lice, Polyplax asiatica and Polyplax spinulosa: intra-genus variation in fragmentation pattern and a possible link between the extent of fragmentation and the length of life cycle

Figure 1

PCR amplification of the mitochondrial (mt) minichromosomes of the Polyplax rat lice. (A) Lane 1: GeneRuler®100 bp DNA Ladder (Thermo Scientific). Lane 2: PCR amplicons generated with primer pair 57F-57R that spans the coding region of each mitochondrial (mt) minichromosome of Polyplax asiatica. Lane 4: PCR amplicons generated with primer pair 301F-301R that spans the coding region of each mt minichromosome of Polyplax spinulosa. (B) PCR verification of the mt minichromosomes of Po. asiatica. Lane 1 and 13: 500 bp DNA Ladder (TIANGEN). Lane 2–12: PCR amplicons from the 11 minichromosomes of Po. asiatica: atp8-atp6, trnE-cob-trnI, cox1-trnL 2 (taa), trnD-trnY-cox2-nad6, trnR-nad4L-cox3-trnA, trnS 1 (tct)-trnS 2 (tga)-nad1-trnT-trnG-nad3-trnW, trnQ-nad2-trnN-trnP, trnK-nad4-trnF, trnH-nad5, rrnS-trnC, trnM-trnL 1 (tag)-rrnL-trnV. (C) PCR verification of the mt minichromosomes of Po. spinulosa. Lane 1 and 13: 500 bp DNA Ladder (TIANGEN). Lane 2–12: PCR amplicons from the 11 minichromosomes of Po. spinulosa: atp8-atp6, trnE-cob-trnI, cox1-trnL 1 (tag), trnT-trnD-trnY-cox2-nad6-trnA, trnR-nad4L-trnP-cox3, nad1-trnG-nad3-trnW, trnQ-nad2-trnN, trnK-nad4, trnH-nad5-trnF, trnS 1 (tct)-trnS 2 (tga)-rrnS-trnC, trnM-trnL 2 (taa)-rrnL-trnV. Genes from which PCR primers were designed are in bold.

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BMC Genomics

ISSN: 1471-2164

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