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Table 1 Exome capture technology designs

From: Performance comparison of four exome capture systems for deep sequencing

  NimbleGen Agilent Illumina TruSeq Illumina Nextera
Bait type DNA RNA DNA DNA
Bait length range (bp) NP 114-126 95 95
Median bait length (bp) NP 119 95 95
Number of baits NP 554,079 347,517 347,517
Total bait length (Mb) NP 66.48 33.01 33.01
Target length range (bp) 59–742 114–21,747 2–37,917 2–37,917
Median target length (bp) 171 200 135 135
Number of targets 368,146 185,636 201071 201,071
Total target length (Mb) 64.19 51.18 62.08 62.08
Fragmentation method Ultrasonication Ultrasonication Ultrasonication Transposomes
Automation ++ ++ ++ +++
Throughput +++ +++ +++ +++
Flexibility Custom available Custom available   Custom available
Species Human, mouse, 3 plant species Human, mouse, 14 other species custom Human Human
Costs $$ $$ $ $
  1. Some NimbleGen information was not provided, indicated by NP. Relative automation and throughput indicated by “+” symbols, higher number of symbols indicates easy to automate and higher throughput. Relative cost is indicated by “$” symbol, higher “$” symbols indicate the higher price.