Table 1 Exome capture technology designs
From: Performance comparison of four exome capture systems for deep sequencing
NimbleGen | Agilent | Illumina TruSeq | Illumina Nextera | |
|---|---|---|---|---|
Bait type | DNA | RNA | DNA | DNA |
Bait length range (bp) | NP | 114-126 | 95 | 95 |
Median bait length (bp) | NP | 119 | 95 | 95 |
Number of baits | NP | 554,079 | 347,517 | 347,517 |
Total bait length (Mb) | NP | 66.48 | 33.01 | 33.01 |
Target length range (bp) | 59–742 | 114–21,747 | 2–37,917 | 2–37,917 |
Median target length (bp) | 171 | 200 | 135 | 135 |
Number of targets | 368,146 | 185,636 | 201071 | 201,071 |
Total target length (Mb) | 64.19 | 51.18 | 62.08 | 62.08 |
Fragmentation method | Ultrasonication | Ultrasonication | Ultrasonication | Transposomes |
Automation | ++ | ++ | ++ | +++ |
Throughput | +++ | +++ | +++ | +++ |
Flexibility | Custom available | Custom available | Custom available | |
Species | Human, mouse, 3 plant species | Human, mouse, 14 other species custom | Human | Human |
Costs | $$ | $$ | $ | $ |