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Table 1 Data for plant growth rate, key metabolites, relative water content and osmotic potential

From: De novo assembly of red clover transcriptome based on RNA-Seq data provides insight into drought response, gene discovery and marker identification

A

Sampling times

Sensitive (mg day-1)

Tolerant (mg day-1)

P

DW0

33 (22-47)

45 (37-54)

P = 0.119

DW1

147 (107-204)

286 (239-342)

 

DW2

80 (58-109)

336 (269-421)

 

DW3

181 (134-244)

369 (302-450)

 

B

Source of variation

df

MS

P

Pool

1

1.599

<0.001

Covariate

1

0.337

0.003

Residual

17

0.027

 

Subject*Time stratum

   

Time

2

0.196

<0.001

Time*Pool

2

0.179

<0.001

Residual

36

0.0163

 

Total

59

  

C

   

Trait

 

Sensitive

Tolerant

 

RWC

Control

84.2 (81.2-87.2)

85.9 (81.9-89.9)

 

Drought

42.5 (40.0-45.1)

78.0 (72.5-84.5)

OP

Control

-1.09 (-1.20-(-0.99)) MPa

-1.14 (-1.20-(-1.09)) MPa

 

Drought

-3.60 (-4.14-(-3.06)) MPa

-2.44 (-2.86-(-2.03) MPa

Glucose

Control

6.94 (5.24-9.19)

6.01 (4.52-7.98)

Drought

3.80 (2.60-5.55)

6.74 (5.39-8.42)

Fructose

Control

7.49 (5.72-9.81)

6.01 (4.57-7.90)

Drought

4.77 (3.84-5.94)

9.14 (6.64-12.60)

myo-Inositol

Control

3.42 (2.76-4.26)

3.29 (2.90-3.74)

Drought

2.46 (2.16-2.82)

3.28 (2.79-3.86)

Malate

Control

14.6 (10.9-19.5)

17.9 (12.8-24.9)

Drought

27.6 (20.6-36.9)

22.2 (20.1-24.5)

Proline

Control

ND

ND

Drought

8.51 (6.28-10.74)

5.00 (2.52-7.48)

Pinitol

Control

17.1 (14.0-20.8)

17.1 (14.3-20.4)

Drought

120.9 (110.4-132.3)

109.6 (99.7-120.5)

  1. A: Plant growth rate and 95% confidence intervals (CI) (in brackets) in the sensitive and tolerant pool. DW0 is growth rate at the onset of the drought stress treatment, DW1 is after 16 days, DW2 is at the end of the drought period (~40 days), and DW3 is growth rate 29 days after the end of the drought stress treatment (recovery). B: ANOVA table for the growth rate data (log10 transformed data). The analysis was performed as a repeated measures design with the DW0 (control) as a co-variate. The P value next to the DW0 data indicates the significance value for the co-variate only. C: Mean values and 95% CI for relative water content (RWC) (%), osmotic potential (OP), and 6 metabolites mg (gDW)-1; ND – below detection limit.
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BMC Genomics

ISSN: 1471-2164

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