Figure 4

Quantitative assessment of lncRNAs in the heart. Expression of the top 10 lncRNAs identified as differentially expressed between MI and sham-operated mice in microarray experiments was quantified using quantitative RT-PCR, first (A) in the derivation group of 8 mice (4 sham and 4 MI), and then (B) in an independent validation group of 16 mice (8 sham and 8 MI). LncRNAs expression is shown relative to GAPDH (log scale). *P < 0.05; #P < 0.001 vs. sham-operated mice. (C) Time-course analysis of MIRT1 and MIRT2 in 21 additional mice subjected to coronary ligation and sacrificed after 1, 3, 6, 16, 24, 48, and 72 hours (n = 3 per time-point). *P < 0.05; #P < 0.001 vs 1 h time-point.