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Figure 5 | BMC Genomics

Figure 5

From: Identification of candidate long non-coding RNAs in response to myocardial infarction

Figure 5

Localisation of MIRT1 in the heart. (A) In situ hybridization revealed that MIRT1 was present mostly in the remote area of the left ventricle, with higher expression observed in MI mice. Representative pictures from 3 sham and 3 MI mice are shown. Arrowheads point to areas with high expression of MIRT1. LV: left ventricle; RV: right ventricle. (B) In situ hybridization coupled to immunostaining revealed that MIRT1 was expressed by cardiac fibroblasts. Sarcomeric alpha-actinin was used to stain cardiomyocytes, vimentin was used for fibroblasts, and the pan-leukocyte antigen CD45 was used for leukocytes. MIRT1 staining appears in green color and is indicated by white arrow heads. Sarcomeric alpha-actinin, vimentin and CD45 appear in red color. Nuclei are blue. Negative control was performed by omission of primary antibody. Magnification: x400.

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