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Table 1 Summary of yeast two-hybrid results

From: Interaction specificity and coexpression of rice NPR1 homologs 1 and 3 (NH1 and NH3), TGA transcription factors and Negative Regulator of Resistance (NRR) proteins

 

B42AD fusion

LexA fusion

NH1

NH2

NH3

NH4

NH5

B42AD

rTGA2.1

+++

++

+++

+++

+

–

rTGA2.2

+++

++

+++

+++

++

–

rTGA2.3

+++

++

+++

+++

+++

–

rLG2

+++

++

+++

+++

+++

–

TGAL1

+++

+++

+++

+++

+++

–

TGAL2

+++

+++

+++

+++

+

–

TGAL4

+++

+++

+++

+++

+++

–

TGAL5

+++

+++

+++

+++

+++

–

TGAL6

+++

++

+++

+++

++

–

TGAL7

++

+++

+++

+++

+++

+

TGAL8

+++

+++

+++

+++

+++

–

TGAL9

±

±

±

+++

+++

–

TGAL11

+++

++

+++

+++

+++

–

LexA

–

–

+

+

+

 
  1. Rice TGA proteins are fused to LexA and NH proteins fused to B42AD. Protein interactions between the two families were tested and semi-quantitative results recorded. At least three independent yeast colonies were included for each pair of proteins. Strong interactions as judged by dark blue colors developed from X-gal are shown as” +++” and no interactions as judged by white colors indicated as “−”. A subscript “+” indicates a slightly lighter blue color than a regular “+”.
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