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Table 3 Summary of split YFP results

From: Interaction specificity and coexpression of rice NPR1 homologs 1 and 3 (NH1 and NH3), TGA transcription factors and Negative Regulator of Resistance (NRR) proteins

YFPC fusion

YFPN fusion

YFPN

rTGA2.1

rTGA2.2

rTGA2.3

rLG2

TGAL1

TGAL2

TGAL4

TGAL5

TGAL6

TGAL7

TGAL8

TGAL9

TGAL11

NH1

±

+++

+++

+++

+++

++ +

+++

+++

++

++

+ +

+ +

+ +

++

NH2

±

NH3

+++

++ +

+++ a

+++ a

++

+++ a

+++ a

++

++

+

+

±

++

NH4

++

++

++

++

++

++

++

++

++

+ +

+ +

+ +

++

NH5

+ +

+ +

+ +

++

+

+ +

+ +

+ +

+

+

±

+

YFPC

 

+

+

+

+

±

±

±

  1. Rice TGA proteins are fused to YFPN and NH proteins fused to YFPC. Interactions between the two families were tested and semi-quantitative results recorded. Strong interactions as judged by bright YFP fluorescence are shown as” +++” and no interactions as judged by lack of YFP fluorescence indicated as “−”. “a” indicates that signals showed in fewer cells compared to other combinations. A subscript “+” indicates a slightly weaker YFP signal than a regular “+”.