Skip to main content

Table 9 Summary of bridged split YFP results

From: Interaction specificity and coexpression of rice NPR1 homologs 1 and 3 (NH1 and NH3), TGA transcription factors and Negative Regulator of Resistance (NRR) proteins

 

YFPN fusion

YFPC fusion

 

rTGA2.1

rTGA2.2

rTGA2.3

rLG2

TGAL2

TGAL4

YFPN

YFPC

NH1ox

 

NH2ox

 

NH3ox

 

NH4ox

 

NH5ox

 

NRR

+

 

NH1ox

+++

+++

+++

+++

+++

+++

NH2ox

±

±

+

±

+

NH3ox

++

++

++

++

++

++

±

NH4ox

+

NH5ox

±

RH1

±

±

±

±

+

 

NH1ox

+++

+++

+++

+++

+++

+++

±

NH2ox

±

±

±

+

+

NH3ox

+++

+++

+++

++/+

+++

+++

±

NH4ox

±

+

+

±

±

+

NH5ox

±

±

+

RH2

±

+

+

+/++

 

NH1ox

+

+

++

+

+++

++

NH2ox

++

+++

+++

++

+++

++

NH3ox

+++

++

+++

+++

+++

+++

NH4ox

±

±

±

±

±

±

NH5ox

RH3

 

NH1ox

+++

+++

+++

+++

+++

+++

NH2ox

+++

+++

+++

+

+++

NH3ox

+

±

+

NH4ox

NH5ox

  1. Rice TGA proteins are fused to YFPN and RH proteins fused to YFPC. Each NH protein was co-introduced with the YFPN and YFPC fusion proteins into protoplasts. Indirect interaction between a TGA and a RH protein, bridged by a NH protein, reconstitutes YFP fluorescence. Semi-quantitative results were recorded. Bright YFP fluorescence are shown as” +++” and lack of YFP fluorescence indicated as “−”.