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Table 6 Scoring system for liver steatosis in individual sections

From: Effects of functional feeds on the lipid composition, transcriptomic responses and pathology in heart of Atlantic salmon (Salmo salar L.) before and after experimental challenge with Piscine Myocarditis Virus (PMCV)

Score

Type of changes

0

No pathological findings or negligible number of inflammatory cells present.

0.1-0.9

Increasing number of inflammatory cells per foci and moderate myocyte necrosis per foci.

1

One or a few distinct focal lesions with cardiomyocyte necrosis, and distinct infiltration of leukocytes (1-5 per foci)

1.1-1.9

Increasing number of lesion individually increasing in size per focus

2

Several distinct lesions and increased number of leukocytes

2.1-2.9

Multifocal myonecrosis with increasing number of inflammatory cells

3

Multifocal to confluent lesions and moderate to severe increase in number of leukocytes

3.1-3.9

Increasing number of foci towards diffuse lesions. Inflammation increases as do endothelial hyperplasia.

4

Severe confluent lesions comprising >75% of the tissue and massive leukocyte infiltration

  1. The changes observed by histological examination have been indicated on a visual analog scale (0-4) based on the criteria given above.