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Table 1 Summary of mapping of RNAseq data used to analyze allele specific expression in F1 cross of B and D

From: Allele-specific expression and eQTL analysis in mouse adipose tissue

Cross

B × D

D × B

B × D

D × B

Sex

Female

Female

Male

Male

Animals per pool

3

2

3

3

Total reads

233,502,200

207,580,666

249,032,306

169,565,576

Reads passing QC

194,608,754

176,900,074

202,559,286

149,205,688

(%)

(83.34%)

(85.22%)

(81.34%)

(87.99%)

Mapped reads

153,220,639

138,009,910

144,153,858

107,732,312

(%)

(78.73%)

(78.02%)

(71.17%)

(72.2%)

Reads mapping to known SNPs (%)

4,761,915

4,598,505

4,617,955

3,733,381

Reads mapping to exonic SNPs (%)*

(85.5%)

(87.64%)

(87.08%)

(88.48%)

B to D mapping ratio (independent SNPs)

1.17 (1.09)

1.21 (1.15)

1.15 (1.08)

1.16 (1.08)

Covered SNPs

201,718

180,513

179,535

153,854

Covered Genes

9,877

9,679

9,796

9,492

  1. *B and D refer to C57BL/6J and DBA/2J strains that differ at 1,834,754 SNPs in 17,002 genes. However, vast majority of these SNPs (>95%) are annotated as intronic, and therefore not necessarily expected to be covered by RNAseq. For simplicity sake, we call all 80,652 non-intronic SNPs “exonic”, while some of them fall within the untranslated regions and are not exonic per se.