Figure 8

Experimental confirmation of VBs as recombination blocks. (A) Validation of the recombination frequencies of 614 F4 RILs on chromosome 8. The top two lanes depict chromosome 8 of the Daepoong and Hwangkeum cultivars. The black and gray sections in the lanes represent the dVBs and sVBs, respectively. The red vertical lines indicate the locations of the small indel markers that were used for the PCR validations. The numbers at the bottom represent the counts of recombination events that occurred in the 614 tested RILs. (B) Genetic and physical linkage maps and resulting recombination rates for chromosome 6. The recombination rates were calculated using 20 indel markers by mapping 614 RILs, through which the VB map was constructed. DP, Daepoong; HK, Hwangkeum.