Identification of mechanosensitive genes during skeletal development: alteration of genes associated with cytoskeletal rearrangement and cell signalling pathways

Table 2 Fold change in expression level of selected genes in Pax3 ^Spd / ^Spd mutant humeri compared to control humeri revealed by Microarray, RNA seq and qRT-PCR

Gene symbol	Gene name	Regulation status	Microarray (Fold change)	RNA-seq (Fold change)	qRT-PCR (Fold change)
Fgf4	Fibroblast growth factor 4	Down-regulated	41.05	#No numerical value	35.24^***
Cilp	Cartilage intermediate layer protein, nucleotide pyrophosphohydrolase	Down-regulated	9.73	9.76	11.3^***
Rxrg	Retinoid X receptor gamma	Down-regulated	7.05	12.29	5.25^**
Dll1	Delta-like 1	Down-regulated	2.21	3.16	1.78^*
Spp1	Secreted phospoprotein 1	Down-regulated	5.23^(ns)	2.9	2.59^*
Vstm2a	V-set and transmembrane domain containing 2A	Up-regulated	8.56	3.09	14.17^**
Figf	c-fos induced growth factor	Up-regulated	3.52	3.17	4.06^**
Fgf10	Fibroblast growth factor 10	Up-regulated	2.48	2.14	3.28
Sfrp2	Secreted frizzled-related protein 2	Up-regulated	2.62	2.09	3.38^*

#No numerical value for fold change as no transcripts detected in the mutant.
***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 indicates significance of differential expression from qRT-PCR.
p ≤ 0.05 for all microarray and RNA-seq differences except Spp1 in the Microarray (ns).

ISSN: 1471-2164