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Table 2 Fold change in expression level of selected genes in Pax3 Spd / Spd mutant humeri compared to control humeri revealed by Microarray, RNA seq and qRT-PCR

From: Identification of mechanosensitive genes during skeletal development: alteration of genes associated with cytoskeletal rearrangement and cell signalling pathways

Gene symbol Gene name Regulation status Microarray (Fold change) RNA-seq (Fold change) qRT-PCR (Fold change)
Fgf4 Fibroblast growth factor 4 Down-regulated 41.05 #No numerical value 35.24***
Cilp Cartilage intermediate layer protein, nucleotide pyrophosphohydrolase Down-regulated 9.73 9.76 11.3***
Rxrg Retinoid X receptor gamma Down-regulated 7.05 12.29 5.25**
Dll1 Delta-like 1 Down-regulated 2.21 3.16 1.78*
Spp1 Secreted phospoprotein 1 Down-regulated 5.23(ns) 2.9 2.59*
Vstm2a V-set and transmembrane domain containing 2A Up-regulated 8.56 3.09 14.17**
Figf c-fos induced growth factor Up-regulated 3.52 3.17 4.06**
Fgf10 Fibroblast growth factor 10 Up-regulated 2.48 2.14 3.28
Sfrp2 Secreted frizzled-related protein 2 Up-regulated 2.62 2.09 3.38*
  1. #No numerical value for fold change as no transcripts detected in the mutant.
  2. ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 indicates significance of differential expression from qRT-PCR.
  3. p ≤ 0.05 for all microarray and RNA-seq differences except Spp1 in the Microarray (ns).