Figure 4

miRNA-target prediction validation and expression. Using TargetScan, potential targets of miR- 6715-3p and miR-6715-5p were identified, based on the complimentarily of the seed region and 6-7 nt sequence of the target mRNA. To test the possibility of miR-target regulation, luciferase assays were performed. A. Protocadherin19 as a potential target of miR-6715-5p. Wild-type 3’UTR had approximately 65% of luciferase activity as compared to the mutant. (*) P < 0.05. B. Vezatin as a potential target of miR-6715-3p. Wild-type 3’UTR had approximately 45% of luciferase activity as compared to the mutant (*) P < 0.05. C. Activator for the Rho-type GTPases Arhgap12 as a potential target of miR- 6715-3p. Wild-type 3’UTR had approximately 85% of luciferase activity as compared to the mutant. (*) P < 0.05. D. Scn8a is not a direct target for miR-6715-3p, as the luciferase activity was not reduced.