Figure 4

Characterization of miRNAs using NGS. (A) Y-axis represents proportion of total reads. Labels:”miRs”-Reads mapping to miRNAs from rat, mouse and human, “Exons”-Reads mapping to rat exons, “Other Non-Coding RNA”-Reads mapping to Non-coding RNA (except miRNAs), “Unaligned”-Reads that could not be aligned to rat genome. (B) Y-Axis represents proportion of the total reads that map to all rat miRNAs (mature + isomiRs). “Identical”-Reads that are identical to miRNAs, “Perfect Matches”- Reads that are shorter than miRNAs, “1 Extra Base”-Reads that have an extra base on 3’ and 5’, “1 Substitution”- Reads that have a substitution on 3’ and 5’, “2 Extra Bases”-Reads that have 2 extra bases on 3’ and 5’, “1 Mismatch”-Reads that have at most 1 mismatch (does not include the previous 1 substitution/deletion/insertion), “PremiRs”-Reads that map the precursor miRNA. (C) Y-Axis gives the proportion of all miRNAs (rat + human + mouse) with maximum value of 1. The rat, mouse and human miRNAs include mature + isomiRs including precursor miRNAs. (D) Hierarchical clustering of the samples. Spearman correlation coefficient was used and the method used was “complete”, using the maximal separation.