Table 2 Urine samples from control (samples 1–7) and gentamicin treated rats (samples 32, 33, 39) were used for RNA isolation using the miRNeasy (Qiagen) extraction kit and analyzed for quality and quantity before NGS analysis
Sample | Gentamicin (mg/kg) | Conc. (ng/ul) | OD 260/280 | OD 260/230 | RIN |
|---|---|---|---|---|---|
1 | 0 | 26.4 | 1.48 | 0.26 | 2.0 |
2 | 0 | 42.7 | 1.57 | 0.29 | 2.6 |
3 | 0 | 41.1 | 1.53 | 0.2 | 2.5 |
4 | 0 | 31.1 | 1.5 | 0.1 | 2.2 |
5 | 0 | 41.5 | 1.61 | 0.22 | 2.6 |
6 | 0 | 28.0 | 1.51 | 0.23 | 1.8 |
7 | 0 | 54.1 | 1.65 | 0.32 | 5.4 |
32 | 50 | 44.9 | 1.71 | 0.57 | 2.6 |
33 | 50 | 46.3 | 1.6 | 0.18 | 2.5 |
39 | 50 | 56.9 | 1.66 | 0.7 | 2.6 |