Figure 3

Differentially expressed miRNAs in ESCs and reprogrammed cells. A. Venn Diagram shows the number of differentially expressed miRNAs in ESCs and the three reprogrammed cell types. The top 50 miRNAs that are differentially expressed between ESCs and each reprogrammed cell type are represented in each circle. The intersection of the three circles indicates 34 miRNAs that are commonly differentially expressed between ESCs and reprogrammed cells. B. Heatmap shows the 34 common reprogrammed cell-specific miRNAs. Except for miR-294, 295, 182, 183, mentioned above, the expression of the remaining miRNAs was similar in ESCs and MEFs. These miRNAs were grouped into two major classes indicated by green and red sidebars. The expression level of the green class of miRNAs was lower in reprogrammed cells than in ESCs and MEFs and the expression level of the red class was higher in reprogrammed cells than in ESCs and MEFs.