Figure 5
Expression pattern analysis in gonad samples of P. margaritifera . 3D Score plot using the first 3PCs identified by principal component analysis of all 1,993 contigs in the 36 individual oyster gonads (A). Hierarchical clustering using Spearman’s correlation on all individual gonad samples (B). Samples are divided into 2 main clusters based on their contig expression pattern, discriminating male and female gonads. Sky blue/pink or (M/F)E: male/female at early stage; cyan/magenta or (M/F)I: male/female at intermediate stage; blue/red or (M/F)M: male/female at mature stage; darkblue/darkpink or (M/F)R: male/female at stage of regression; green or Inv: gonads in sexual inversion; and grey or Und: gonad sex is undetermined.