Figure 7
Highlighted expression profiles of eight reproductive genes in P. margaritifera gonad samples. RNAseq normalized expression (estimatesizefactor) profiles of some genes determined as differentially expressed over the ten different gonadic categories (sequenced samples). Vertical bars represent standard deviation for RNAseq data (n = 4 except for the undetermined and inversion categories where n = 2). Expression profiles are shown for specific genes expressed in female gonads: forkhead box protein l2 (pmarg-foxl2, Contig_43072, cluster 7), vitellogenin-6 (vit-6, Contig_15150, cluster 8), fatty acid synthase (fasn, Contig_22662, cluster 8), endoribonuclease dicer (dicer1, Contig_28823, cluster 9), superoxide dismutase 1 (soda, Contig_25451, cluster 7) and dna (cytosine-5)-methyltransferase 1 (dnmt1, Contig_674, cluster 9); three specific genes expressed in male gonads: doublesex- and mab-3-related transcription factor-like (pmarg-dmrt, Contig_639, cluster 4), testis-specific serine/threonine-protein kinase 1 (tssk1, Contig_4767, cluster 5) and f-box only protein 39 (fbxo39, Contig_3220, cluster 3); and three genes expressed in male and female gonads with a higher level in the male: sex-determining protein fem-1-like (pmarg-fem1-like, Contig_1317, cluster 2), g2/mitotic-specific cyclin-b3 (ccnb3, Contig_1166, cluster 2) and dynein intermediate chain 3, ciliary (dyi3, Contig_3511, cluster 1). Development stage (E: early, I: intermediate, M: mature, R: regression) and sex (M: male, F: female, Und: undetermined sex, Inv: in sexual inversion) are indicated at the bottom of each figure. Different letters indicate statistically significant differences (DESeq, padj < 0.001).