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Figure 8 | BMC Genomics

Figure 8

From: Gonad transcriptome analysis of pearl oyster Pinctada margaritifera: identification of potential sex differentiation and sex determining genes

Figure 8

Validation of the RNAseq quantification data using q-RT-PCR. Fourteen genes were selected from differentially and non-differentially expressed contigs. There were 11 genes showing different expression levels depending on the sex and the maturity of the oysters: expression in both sexes (two genes from cluster 1: tex11 [Contig_1600] and dnah6 [Contig_2426]; and one gene from cluster 2: ccnb3 [Contig_1166]), higher expression in males (one gene from cluster 4: pmarg-dmrt [Contig_639]; and two genes from cluster 5: tssk1 [Contig_4767] and mprb [Contig_50490]), and higher expression in females (2 genes from cluster 7: sox2 [Contig_37098] and mos [Contig_51755]; two genes from cluster 8: vit-6 [Contig_15150] and pnliprp1 [Contig_23348]; and one gene from cluster 9: pbx4 [Contig_57857]). The three remaining genes, not differentially expressed but annotated as reproductive genes, were pmarg-dmrt2 [Contig_44478], dax1 [Contig_3226], and vasa [Contig_2898]. Their relative expression (to ef1 and gapdh1) quantified by q-RT-PCR on samples used for sequencing were compared with those obtained using the RNAseq approach (normalized expression, estimatesizefactor) (RS= 0.86; p < 0.00001).

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