Table 1 Summary statistics of P. margaritifera gonad transcriptome sequencing, assembly and annotation
| Â | Number | Percentage |
|---|---|---|
Reads | Â | Â |
Total number | 2125798302 | Â |
Number of base pairs | 214705628502 | Â |
Length (bp) | 100 | Â |
Average insertion size (bp) | 198 | Â |
Assembly | Â | Â |
Number of contigs | 70147 | Â |
Number of base pairs | 90799729 | Â |
Number of GC base pairs | 33298120 | 36.7% |
Mapping rate of reads | Â | 91.8% |
Average coverage of contigs (reads) | 773.3 | Â |
Average coverage of contigs (rpkm) | 9.8 | Â |
Average length of contigs (bp) | 1294 | Â |
N50 of contigs (bp) | 1948 | Â |
Longest contig (bp) | 17424 | Â |
Shortest contig (bp) | 100 | Â |
Annotation | Â | Â |
Unannotated contigs | 26531 | 37.8% |
Annotated contigs | 43616 | 62.2% |
Best hits (Hits): | Â | Â |
- P. fucata proteins | 23522 (33448) | 53.9% (76.7%) |
- C. gigas proteins | 9517 (23154) | 21.8% (53.1%) |
- Swissprot | 362 (18736) | 0.8% (43%) |
- RefSeqProtein | 2205 (22261) | 5.1% (51%) |
- RefSeq RNA | 177 (5265) | 0.4% (12.1%) |
- Otherdatabases | 7833 (19131) | 18% (43.9%) |