Table 4 Differentially expressed proteins in highly and weakly pathogenic N. fowleri, as identified via 1D gel electrophoresis in combination with nano-LC MS/MS

Q3SZP7

Villin-1

Bos taurus

16.87

10.19

Q55585

Probable succinate-semialdehyde dehydrogenase [NADP (+)]

Synechocystis sp. PCC 6803 substr. Kazusa

9.09

11.63

Q4UB16

Ras-related protein Rab-1

Theileria annulata

8.88

9.39

P08799

Myosin II heavy chain

Dictyostelium discoideum

8.03

3.86

Q4R550

Cysteine--tRNA ligase, cytoplasmic

Macaca fascicularis

7.35

4.79

B7MMS8

Gamma-aminobutyraldehyde dehydrogenase

Escherichia coli S88

7.17

7.48

O75382

Tripartite motif-containing protein 3

Homo sapiens

6.43

3.91

Q9V4N3

Cytochrome b5; Short = CYTB5

Drosophila melanogaster

4.46

2.62

P27420

Heat shock 70 kDa protein C

Caenorhabditis elegans

4.14

2.77

Q9N1T2

X-linked retinitis pigmentosa GTPase regulator

Canis lupus familiaris

4.12

4.97

D2VAA9

Methylthioribose-1-phosphate isomerase

Naegleria gruberi

3.91

2.90

Q25544

26S protease regulatory subunit 8 homolog

Naegleria fowleri

3.88

2.50

P54772

Histidine decarboxylase

Solanum lycopersicum

2.91

8.22

P10733

Severin

Dictyostelium discoideum

2.88

2.73

Q1HPW4

Eukaryotic translation initiation factor 3 subunit I

Bombyx mori

2.85

2.23

Q54BW4

Circularly permutated Ras protein 2

Dictyostelium discoideum

2.46

3.79

Q12965

Unconventional myosin-Ie

Homo sapiens

2.30

3.56

P34552

Apoptosis-linked gene 2-interacting protein X 1

Caenorhabditis elegans

2.30

2.21

Q54K50

Phospholipase D Y

Dictyostelium discoideum

2.22

2.19

Q5TJ55

Formin-D

Dictyostelium discoideum

2.18

8.77

Q1ZXF7

GDP-mannose 4,6 dehydratase

Dictyostelium discoideum

2.15

2.44

Q8IV36

Protein HID1

Homo sapiens

2.07

2.71

P13629

Periplasmic [Fe] hydrogenase large subunit

Desulfovibrio oxamicus (strain Monticello)

−2.14

−2.86

P08964

Myosin-1

Saccharomyces cerevisiae S288c

−2.25

−5.91

P51824

ADP-ribosylation factor 1

Solanum tuberosum

−2.38

−6.44

A7HBL7

Elongation factor Tu

Anaeromyxobacter sp. Fw109-5

−2.46

−2.42

Q9CR62

Mitochondrial 2-oxoglutarate/malate carrier protein

Mus musculus

−2.56

−3.14

Q1ZXF1

Probable enoyl-CoA hydratase, mitochondrial

Dictyostelium discoideum

−2.69

−4.44

F4P6T0

Ubiquinol oxidase, mitochondrial

Batrachochytrium dendrobatidis JAM81

−2.97

−5.61

P77735

Uncharacterized oxidoreductase YajO

Escherichia coli K-12

−3.06

−2.15

Q889U1

Single-stranded DNA-binding protein

Pseudomonas syringae pv. tomato str. DC3000

−3.19

−3.39

P48375

12 kDa FK506-binding protein

Drosophila melanogaster

−3.55

−2.95

Q2G8K9

Elongation factor Ts

Novosphingobium aromaticivorans DSM 12444

−3.56

−2.27

Q7S6S4

Mitochondrial import inner membrane translocase subunit tim-16

Neurospora crassa OR74A

−3.67

−2.80

P54168

Uncharacterized protein YpgQ

Bacillus subtilis subsp. subtilis str. 168

−3.9

−3.05

O42899

Protein sco1

Schizosaccharomyces pombe 972 h-

−3.91

−7.12

Q97FZ9

Rubrerythrin-1

Clostridium acetobutylicum ATCC 824

−4.94

−2.06

Q9P7M0

ABC1 family protein C21C3.03, mitochondrial

Schizosaccharomyces pombe 972 h-

−5.73

−2.62

P42730

Chaperone protein ClpB1

Arabidopsis thaliana

−6.29

−4.14

P54872

Hydroxymethylglutaryl-CoA synthase A

Dictyostelium discoideum

−6.3

−3.2

Q60597

2-oxoglutarate dehydrogenase, mitochondrial

Mus musculus

−8.13

−2.87

P42125

Enoyl-CoA delta isomerase 1, mitochondrial

Mus musculus

−8.93

−2.44

O67589

Aspartate--tRNA ligase

Aquifex aeolicus VF5

−20.48

−11.26