Figure 1

The phylogenetic tree of C. roseus (red dot), A. trichopoda (inverted purple triangle), A. thaliana (green square), and O. sativa (blue diamond) was constructed using the Neighbor-Joining method with P-distance substitution model, pairwise-deletion, and a bootstrap value of 2000. WRKY domain alignment was performed with ClustalW. Proteins used as an outgroup are indicated by a teal triangle. WRKY TFs from six additional medicinal species, A. annua (AaWRKY1), C. japonica (CjWRKY1), G. arboreum (GaWRKY1), H. brasiliensis (HbWRKY1), P. quinquefolius (PqWRKY1), and T. chinensis (TcWRKY1), were included. WRKY TFs involved in regulating secondary metabolism are indicated with a black dot.