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Figure 1 | BMC Genomics

Figure 1

From: Genome sequences characterizing five mutations in RNA polymerase and major capsid of phages ϕA318 and ϕAs51 of Vibrio alginolyticus with different burst efficiencies

Figure 1

Different characteristics between phages ϕA318 and ϕAs51. (A) The plaque sizes within 16 h after phage infection at 25°C. (B) Transmission electron micrographs of phage particles. Virions were negatively stained with uranyl acetate. The bars represent a length of 100 nm. (C) One-step growth curves of phages in V. alginolyticus at 25°C for ϕA318 and 37°C for ϕAs51. The burst sizes were calculated by the amplifying ratio of Pt/P0 (phage titer at the plateau phase is divided by the inoculating phage titer). (D) Thermal stability of phages at various temperatures one hour. Samples were taken to determine the titer of the surviving particles and to calculate the percentage of infectious phage. In the stationary phase, standard errors (SE) for each phage were consistent at 16-20% (SE/mean), while the standard errors (SE) were approximate 16-72% (SE/mean) during the exponential phase.

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BMC Genomics

ISSN: 1471-2164

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