TY - JOUR AU - Naeem, Haroon AU - Wong, Nicholas C. AU - Chatterton, Zac AU - Hong, Matthew K. H. AU - Pedersen, John S. AU - Corcoran, Niall M. AU - Hovens, Christopher M. AU - Macintyre, Geoff PY - 2014 DA - 2014/01/22 TI - Reducing the risk of false discovery enabling identification of biologically significant genome-wide methylation status using the HumanMethylation450 array JO - BMC Genomics SP - 51 VL - 15 IS - 1 AB - The Illumina HumanMethylation450 BeadChip (HM450K) measures the DNA methylation of 485,512 CpGs in the human genome. The technology relies on hybridization of genomic fragments to probes on the chip. However, certain genomic factors may compromise the ability to measure methylation using the array such as single nucleotide polymorphisms (SNPs), small insertions and deletions (INDELs), repetitive DNA, and regions with reduced genomic complexity. Currently, there is no clear method or pipeline for determining which of the probes on the HM450K bead array should be retained for subsequent analysis in light of these issues. SN - 1471-2164 UR - https://doi.org/10.1186/1471-2164-15-51 DO - 10.1186/1471-2164-15-51 ID - Naeem2014 ER -